| Next-generation high-density self-assembling functional protein arrays | View |
| NAPPA arrays: expression cloning, array creation, protein expression and detection | View |
| Growth and preparation of Salmonella cells fom LB media for proteolytic digestion | View |
| FTICR Data Analysis | View |
| Growth and preparation of Salmonella cells from host RAW cells | View |
| Analysis of peptide samples by capillary liquid chromatography-mass spectrometry | View |
| PNNL NIAID MS Sample Naming Convention | View |
| Entry vector cloning: primer design, ORF cloning, sequence analysis | View |
| Comparative Proteomics of Human Monkeypox and Vaccinia Intracellular Mature
and Extracellular Enveloped Virions | View |
| Murine Macrophage cDNA microarray experiment protocol | View |
| Affymetrix microarray experiment protocol | View |
| ADRP Cloning, Expression and Structure Methods | View |
| NMRNps Cloning, Expression and Structure Methods | View |
| NSP10 Cloning, Expression and Structure Methods | View |
| Orf9aNterm Domain Cloning, Expression and Structure Methods | View |
| PLP Cloning, Expression and Structure Methods | View |
| Infection of RAW264 macrophages with Brucella abortus | View |
| Immunomagnetic plasma membrane isolation from Brucella-infected RAW264 cells | View |
| MHC peptides isolation from RAW264 cell lysate for MS analysis | View |
| Preparation of infected RAW264 cell lysate for MHC isolation | View |
| Solubilization and processing of bacterial cell envelopes for MS analysis | View |
| Solubilization and processing of intact bacteria for MS analysis | View |
| Solubilization and processing of phagosomes for MS analysis | View |
| Solubilization and processing of plasma membrane for MS analysis | View |
| Preparation of non-infected RAW264 cell lysate for MHC isolation | View |
| LTQ Ion Trap (QIT) mass spectrometer protocol | View |
| An automated process for the large-scale identification of protein-protein interactions | View |
| Supplemental and modified procedures for Shi et al. JBC 2006 | View |
| Sequest analysis | View |
| Growth and preparation of Salmonella cells from LB media following MgM shock | View |
| Global and soluble sample preparation from BSL 2 and 3 prokaryotic and viral organisms | View |
| BSL2 and 3 Sample Preparation from Prokaryotic and Viral Organisms Using Rapigest | View |
| Strong cation exchange (SCX) fractionation of peptides using HPLC | View |
| Low Molecular Weight Peptide/Protein Sample Preparation from BSL2 Prokaryotic Organisms | View |
| BSL3 Sample Preparation from Orthopox Virus Using Rapigest | View |
| Immunization and bleeding schedule of rabbits | View |
| The ELISA test is performed to determine the titer of a specific polyclonal antibody serum | View |
| Conjugation method of peptide with KLH (for antibody production) or BSA (for ELISA) | View |
| Protocol to Amplify an Activation Domain Library (Adl) >From the Permanent Frozen Stock | View |
| Reagent for Activation Domain Library Activation | View |
