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Technology Description	The utility of accurate mass and LC elution time information in the analysis of complex proteomes.
Category	Technology
PRC	Pacific Northwest National Laboratory
PubMed ID	15979333
Author	Norbeck AD, Monroe ME, Adkins JN, Anderson KK, Daly DS, Smith RD
Publication Description	The combination of mass and normalized elution time (NET) of a peptide identified by liquid chromatography-mass spectrometry (LC-MS) measurements can serve as a unique signature for that peptide.
Methodology	The specificity of an LC-MS measurement depends upon the complexity of the proteome (i.e., the number of possible peptides) and the accuracy of the LC-MS measurements. In this work, theoretical tryptic digests of all predicted proteins from the genomes of three organisms of varying complexity were evaluated for specificity. Accuracy of the LC-MS measurement of mass-NET pairs (on a 0 to 1.0 NET scale) was described by bivariate normal sampling distributions centered on the peptide signatures. Measurement accuracy (i.e., mass and NET standard deviations of ±0.1, 1, 5, and 10 ppm, and ±0.01 and 0.05, respectively) was varied to evaluate improvements in process quality.