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Technology Description	Silica-based monolithic capillary columns with integrated nanoESI emitters have been developed to provide high-quality and robust microSPE-nanoLC-ESI-MS analyses.
Category	Technology
PRC	Pacific Northwest National Laboratory
PubMed ID	17222018
Author	Quanzhou Luo, Jason S. Page, Keqi Tang, and Richard D. Smith
Publication Description	The authors developed a microSPE-nanoLC-ESI-MS platform that uses 10-ím-i.d. silica-based monolithic columns in conjunction with a robust integrated ESI emitter for significantly improved sensitivity in proteomic analysis. In one of the first applications with a linear ion trap MS, the group identified 5510 unique peptides that covered 1443 distinct Shewanella oneidensis proteins from a 300-ng tryptic digest sample in a single 4-h LC-MS/MS analysis.
Methodology	The dominant parameter that influences ESI sensitivity is the liquid flow rate.11-21 The use of lower flow rates decreases the initial charged droplet size and improves ionization efficiency and detection limits. A microSPE-nanoLC-ESI-MS platform that employs a 10-ími.d., silica-based monolithic capillary column with an integrated tapered nanoESI tip was developed and evaluated for application to very small proteomics samples.