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Technology Description	A combined electrodynamic ion funnel and ion trap coupled to an orthogonal acceleration time-of-flight mass spectrometer.
Category	Technology
PRC	Pacific Northwest National Laboratory
PubMed ID	17850113
Author	Yehia Ibrahim, Mikhail E. Belov, Aleksey V. Tolmachev, David C. Prior, and Richard D. Smith
Publication Description	A combined electrodynamic ion funnel and ion trap coupled to an orthogonal acceleration-time-of-flight mass spectrometer was developed and characterized. An order of magnitude increase in sensitivity was observed in the analysis of low concentration peptides mixtures with orthogonal acceleration-time-of-flight mass spectrometry in the trapping mode as compared to the continuous regime. Controlling the ion trap ejection time was found to result in efficient removal of singly charged species and improving signal-to-noise ratio for the multiply charged analytes.
Methodology	Ions from the electrospray source were transmitted through a stainless steel capillary interface,resistively heated to 165 °C, and into an electrodynamic ion funnel that worked at pressure of 1 Torr. The 180° phase-shifted rf fields were applied to adjacent ring-electrodes. Ion transmission through the funnel was improved by superimposing a dc field onto the rf field applied to each electrode.