Technology

Select an Option

Technology Detail

Technology Description	Next-generation high-density self-assembling functional protein arrays
Category	Technology
PRC	Harvard Institute of Proteomics
PubMed ID	18469824
Author	Ramachandran N, Raphael JV, Hainsworth E, Demirkan G, Fuentes MG, Rolfs A, Hu Y, LaBaer J
Publication Description	Harvard Institute of Proteomics developed a high-density self-assembling protein microarray, based on the nucleic acid programmable protein array (NAPPA) concept, to display thousands of proteins that are produced and captured in situ from immobilized cDNA templates.
Methodology	Nucleic acid programmable protein array (NAPPA) allows for functional proteins to be synthesized in situ directly from printed cDNAs just in time for assay4. The proteins are translated using a T7-coupled rabbit reticulocyte lysate in vitro transcription-translation (IVTT) system. The expressed proteins are captured locally with an antibody to a C-terminal glutathione S-transferase (GST) tag on each protein (anti-GST).