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Technology DescriptionTracking humoral responses using self assembling protein microarrays
CategoryTechnology
PRCHarvard Institute of Proteomics
AuthorNiroshan Ramachandran, Karen S. Anderson, Jacob V. Raphael, Eugenie Hainsworth, Sahar Sibani, Wagner R. Montor, Marcin Pacek, JessicaWong, Mariam Eljanne, Martin G. Sanda, Yanhui Hu,Tanya Logvinenko and Joshua LaBaer
Publication DescriptionHarvard Institute of Proteomics presented the development of methods for applying self-assembling protein microarrays and a related method for producing 96-well formatted macroarrays for monitoring the humoral response at the proteome scale.
MethodologyAn alternative method was developed for generating protein microarrays in which printed and anchored fulllength expression cDNAs encoding target proteins fused to a common epitope are expressed and translated locally to produce proteins in situ on the array surface. The fusion proteins are captured using antitag antibodies that are coprinted on the arrays, enabling the presentation of over 3000 individual antigens per slide.
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