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Technology Description	This study demonstrates that high-resolution electron density maps and refined models can be obtained from in situ diffraction of crystals grown in microcapillaries.
Category	Structure
PRC	Scripps Research Institute
PubMed ID	17468785
Author	Maneesh K. Yadav, Cory J. Gerdts, Ruslan Sanishvili, Ward W. Smith, L. Spencer Roach, Rustem F. Ismagilov, Peter Kuhn and Raymon C. Stevens
Publication Description	In situ X-ray data collection has the potential to eliminate the challenging task of mounting and cryocooling often fragile protein crystals, reducing a major bottleneck in the structure determination process. An apparatus used to grow protein crystals in capillaries and to compare the background X-ray scattering of the components, including thin-walled glass capillaries against Teflon, and various fluorocarbon oils against each other, is described.
Methodology	We demonstrate that microcapillary-based microbatch screening offers an alternative method to increase the efficiency of small volumes, and has the advantage of convenient in situ data collection and a potentially significant reduced cost of production.
Resource	http://kuhn.scripps.edu/Lists/Publications/DispForm.aspx?ID=6