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Technology Description	Multidimensional fingerprinting (MDF) utilizes measurable peptide characteristics to identify proteins
Category	Technology
PRC	Caprion Proteomics Inc.
Author	Kossi Lekpor, Marie-Josée Benoit, Heather Butler, Michael Schirm, Daniela Vasilescu, Katherine Bonter, Daniel Chelsky, Patrice Hugo, Joanna Hunter, Gregory Opiteck, Eustache Paramithiotis and Paul Kearney
Publication Description	In this study, 3-D fingerprinting, namely, parent protein molecular weight, peptide mass, and peptide retention time on RPLC, is used to identify 331 differentially expressed proteins between normal and human colon cancer plasma membrane samples. A false discovery rate (FDR) procedure is introduced to evaluate the performance of MDF on the colon cancer dataset. This evaluation establishes a false protein identification rate below 15% for this dataset.
Methodology	This simulation study demonstrates that fingerprinting with three dimensions yields low FDR values even for large queries on the complete human proteome without the need for prior peptide sequencing by tandem mass spectrometry. Specifically, when mass accuracy is 10 ppm or lower, full human proteome searches can achieve FDR values of 10% or less.
Resource	http://www3.interscience.wiley.com/cgi-bin/abstract/114212036