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Project NameMyriad protein to protein interaction through Y2H System
PRCMyriad Genetics
Project DescriptionIdentification of proteins involved in host/pathogen (Homo sapiens, B. anthracis, F. tularensis, Y. pestis, Vaccinia virus, Variola virus) interactions; Identification of biochemical pathways in pathogenic organisms; Identification of proteins binding to domains of membrane-associated proteins; Rapid translation of targets into high-throughput drug screens; Disrupting protein-protein interactions in high-throughput drug screens and elucidating protein-small molecule interactions; Elaboration of signaling and biochemical pathways and extension of biochemical pathways from previously uncharacterized genes.
Experiment DesignDISCLAIMER: The random two hybrid (R2H) assay was intended to identify bacterial proteins that potentially interact with human proteins. This data has served as a base to further validations in the directed yeast two hybrid (Y2H) system. The intention was to use this method as a high throughput screening tool and not to interpret specific interaction data on its own. Bacterial proteins have been nominated for Y2H searching using a statistical model. This data should be considered raw and includes good, bad and self activating searches. Myriad makes no guarantees as to the reproducibility of any interactions in this dataset.
Experiment Data Downloads
FTP download
Request additional experiment data and details from max@myriad.com
Protocols
Protocol to Amplify an Activation Domain Library (Adl) >From the Permanent Frozen Stock
Reagent for Activation Domain Library Activation
Organisms
Homo sapiens, Mus musculus, Yersinia pestis KIM
Contact National Institute of Allergy and Infectious Disease National Institutes of Health National Institutes of Health