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Project NameEvaluation of the Salmonella typhimurium Proteome
PRCPacific Northwest National Laboratory
Project DescriptionTo develop a deeper understanding of the infectious state of S. typhimurium, liquid chromatography-mass spectrometry-based “bottom-up” proteomics was used to globally analyze the proteins present under specific growth conditions
Experiment DesignA histidine-biotin-histidine (HBH) tag is added to specific proteins via recombinant DNA techniques, and the resultant construct is transformed and expressed in the target organism. After cross-linking in vivo with formaldehyde to stabilize protein−protein interactions from the cell context, the bait proteins are isolated under denaturing conditions with the relevant interacting proteins attached via the cross-link and identified by mass spectrometry (MS).
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Organisms
Salmonella typhimurium 14028
Contact National Institute of Allergy and Infectious Disease National Institutes of Health National Institutes of Health